Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2006 Sep;3(9):721-3.
doi: 10.1038/nmeth922.

Nanoscale resolution in GFP-based microscopy

Katrin I Willig  1 , Robert R Kellner, Rebecca Medda, Birka Hein, Stefan Jakobs, Stefan W Hell
Affiliations
Review

Nanoscale resolution in GFP-based microscopy

Katrin I Willig et al. Nat Methods. 2006 Sep.

Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

PubMed Disclaimer

Substances

LinkOut - more resources