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Case Reports
. 2006 Nov;45(11):1072-6.
doi: 10.1002/gcc.20370.

Overexpression of PRDM16 in the presence and absence of the RUNX1/PRDM16 fusion gene in myeloid leukemias

Sawcène Hazourli, Pierre Chagnon, Martin Sauvageau, Raouf Fetni, Lambert Busque, Josée Hébert
Case Reports

Overexpression of PRDM16 in the presence and absence of the RUNX1/PRDM16 fusion gene in myeloid leukemias

Sawcène Hazourli et al. Genes Chromosomes Cancer. 2006 Nov.
No abstract available

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Figures

Figure 1
Figure 1
(A) Standard cytogenetic analysis of leukemic cells with the t(9;22)(q34.1;q11.2) and ?del(21)(q22) (UPN 02H056). (B) Spectral karyotype of leukemic cells with the t(9;22)(q34.1;q11.2) and t(1;21)(p36.32;q22) (UPN 02H056). (C) FISH on metaphases with the t(1;21)(p36.32;q22) (UPN 02H056) using the RUNX1 probe (BAC RP11-299D9) that shows one signal on the normal chromosome 21 and a split signal on the rearranged der(1) and der(21) (long arrows). (D) FISH on metaphases with the t(1;21)(p36.32;q22) (UPN 02H056) using the PRDM16 probe (PAC RP1-163G9) that shows one signal on the normal chromosome 1 (short arrow) and a split signal on the der(1) and der(21) (long arrows).
Figure 2
Figure 2
Schematic representation of RUNX1 and PRDM16 wild-type proteins and the putative chimeric protein RUNX1/PRDM16. Partial nucleotide and amino acid sequences of the two RUNX1/PRDM16 in-frame fusions. Arrows indicate the t(1;21) breakpoints. RHD, runt homology domain; TA, transactivation domain; PRD, PR domain; ZnF, zinc finger domain; PRR, proline-rich domain; RD, repressor domain; AD, acidic domain.
Figure 3
Figure 3
Expression analysis of PRDM16 (exons 9 and 10) using real-time PCR in 105 samples: 4 normal bone marrow (BM) samples, CD34 isolated from normal bone marrow (CD34+), 5 normal peripheral blood (PB) samples, one colon carcinoma cell line (HCT-116), 4 CML-BP derived cell lines: K562, KU812, MC3, MEG01, 14 MDS and MDS/AML, 7 CML-BP (including present case with t(1;21), 02-H056), present CML case in chronic phase (CML-CP, 00-H59), 68 AML (FAB type: M0, M1, M2, M4, M5 of different cytogenetic groups and AML with t(11)(q23), t(15;17), and t(8;21) and with inv(16)). All samples were tested in duplicate and the average values were used for quantification. The graph shows ratios of 1/ΔCt. The horizontal gray line indicates the average ratio in the four normal bone marrow samples (calibrator) and the horizontal dotted lines indicate an arbitrary 1/ΔCt ratio of 0.21 and 0.09 for significant up or down-regulation respectively (or mRNA levels more than 10-fold above or under value detected in normal BM control cells). ΔCt values are: 04-H070: 3.1; 02-H056: 0.3; 06-H006: 3.4; 00-H59: 7.3; 02-H009: 0.2; 02-H075: 3.1; 03-H033: 1.9; 04-H120: 1.7; 04-H006: 0.5; 04-H054: −2.2; 03-H052: 1.1; Average normal BM: 8.0.
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References

    1. Barjesteh van Waalwijk van Doorn-Khosrovani S, Erpelinck C, Lowenberg B, Delwel R. Low expression of MDS1-EVI1-like-1 (MEL1) and EVI1-like-1 (EL1) genes in favorable-risk acute myeloid leukemia. Exp Hematol. 2003;31:1066–1072. - PubMed
    1. Cuenco GM, Ren R. Cooperation of BCR-ABL and AML1/MDS1/EVI1 in blocking myeloid differentiation and rapid induction of an acute myelogenous leukemia. Oncogene. 2001;20:8236–8248. - PubMed
    1. Du Y, Jenkins NA, Copeland NG. Insertional mutagenesis identifies genes that promote the immortalization of primary bone marrow progenitor cells. Blood. 2005;106:3932–3939. - PMC - PubMed
    1. Gribble SM, Reid AG, Roberts I, Grace C, Green AR, Nacheva EP. Genomic imbalances in CML blast crisis: 8q24.12-q24.13 segment identified as a common region of over-representation. Genes Chromosomes Cancer. 2003;37:346–358. - PubMed
    1. Jiang GL, Huang S. The yin-yang of PR-domain family genes in tumorigenesis. Histol Histopathol. 2000;15:109–117. - PubMed

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