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. 1990 Apr;10(4):1805-10.
doi: 10.1128/mcb.10.4.1805-1810.1990.

The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing

M T Huang  1 , C M Gorman
Affiliations

The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing

M T Huang et al. Mol Cell Biol. 1990 Apr.

Abstract

Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSVcat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.

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