Direct gene transfer into mouse muscle in vivo
- PMID: 1690918
- DOI: 10.1126/science.1690918
Direct gene transfer into mouse muscle in vivo
Abstract
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for beta-galactosidase activity was localized to muscle cells following injection of the beta-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.
Similar articles
-
Direct DNA injection into mouse tongue muscle for analysis of promoter function in vivo.Somat Cell Mol Genet. 1993 Mar;19(2):111-22. doi: 10.1007/BF01233527. Somat Cell Mol Genet. 1993. PMID: 8511670
-
Improved cationic lipid formulations for in vivo gene therapy.Ann N Y Acad Sci. 1995 Nov 27;772:126-39. doi: 10.1111/j.1749-6632.1995.tb44738.x. Ann N Y Acad Sci. 1995. PMID: 8546385 Review.
-
Efficient gene transfer into zebrafish skeletal muscle by intramuscular injection of plasmid DNA.Mol Mar Biol Biotechnol. 1997 Jun;6(2):98-109. Mol Mar Biol Biotechnol. 1997. PMID: 9200836
-
In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.Proc Natl Acad Sci U S A. 1990 Dec;87(24):9568-72. doi: 10.1073/pnas.87.24.9568. Proc Natl Acad Sci U S A. 1990. PMID: 2175906 Free PMC article.
-
Vectors with bidirectional reporter genes for studying divergent promoters.Methods Enzymol. 1996;273:319-31. doi: 10.1016/s0076-6879(96)73028-8. Methods Enzymol. 1996. PMID: 8791621 Review. No abstract available.
Cited by
-
Taylor Dispersion Analysis to support lipid-nanoparticle formulations for mRNA vaccines.Gene Ther. 2023 May;30(5):421-428. doi: 10.1038/s41434-022-00370-1. Epub 2022 Nov 1. Gene Ther. 2023. PMID: 36316446 Free PMC article.
-
Profile of Katalin Karikó and Drew Weissman: 2023 Nobel laureates in Physiology or Medicine.Proc Natl Acad Sci U S A. 2024 Feb 27;121(9):e2400423121. doi: 10.1073/pnas.2400423121. Epub 2024 Feb 21. Proc Natl Acad Sci U S A. 2024. PMID: 38381788 Free PMC article. No abstract available.
-
Formation of dsRNA by-products during in vitro transcription can be reduced by using low steady-state levels of UTP.Front Mol Biosci. 2023 Dec 11;10:1291045. doi: 10.3389/fmolb.2023.1291045. eCollection 2023. Front Mol Biosci. 2023. PMID: 38146535 Free PMC article.
-
Chitosans for delivery of nucleic acids.Adv Drug Deliv Rev. 2013 Aug;65(9):1234-70. doi: 10.1016/j.addr.2013.07.005. Epub 2013 Jul 18. Adv Drug Deliv Rev. 2013. PMID: 23872012 Free PMC article. Review.
-
Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals.Mol Ther. 2015 Sep;23(9):1456-64. doi: 10.1038/mt.2015.103. Epub 2015 Jun 8. Mol Ther. 2015. PMID: 26050989 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources