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. 1990 Jan;184(1):100-3.
doi: 10.1016/0003-2697(90)90019-6.

Purification of gramicidin A

C J Stankovic  1 , J M Delfino, S L Schreiber
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Free article

Purification of gramicidin A

C J Stankovic et al. Anal Biochem. 1990 Jan.
Free article

Abstract

A simple chromatographic purification of the naturally occurring ion channel-forming pentadecapeptide gramicidin A (gA) is presented. This procedure allows gA to be isolated in gram quantities from the commercially available mixture of isomers after chromatography on silica gel. The gramicidin A obtained in this manner is greater than 95% pure as determined by 1HNMR, HPLC, and amino acid analysis.

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