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. 2006 Aug;38(4):349-52.
doi: 10.1080/00313020600820807.

Shortcut detection of the vanB gene cluster in enterococci by a duplex real-time PCR assay

D N Adams  1
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Shortcut detection of the vanB gene cluster in enterococci by a duplex real-time PCR assay

D N Adams. Pathology. 2006 Aug.

Abstract

Aim: To develop a robust, simple and rapid method for detection of vanB in enterococci.

Methods: A real-time duplex PCR assay for the simultaneous detection of Enterococcus faecium and vanB resistance genotype in enterococci was developed in conjunction with a simple method for DNA extraction. The assay was tested on 130 fresh plate cultures of clinical isolates of enterococci and other Gram-positive bacteria.

Results: Forty-eight isolates of vanB E. faecium from 32 different patients and three isolates of vanB E. faecalis were detected within 1 hour. All isolates of E. faecium were identified correctly.

Conclusion: This simple method for the detection of resistance mediated by vanB is a potentially useful method that is suitable for use in the diagnostic microbiology laboratory.

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