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. 1990 Mar;8(3):250-1.

Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge

K J Reddy  1 , R Webb, L A Sherman
Affiliations
  • PMID: 1691916

Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge

K J Reddy et al. Biotechniques. 1990 Mar.

Abstract

A procedure for the rapid preparation of cesium-chloride purified RNA from E. coli and the cyanobacterium Synechococcus sp. PCC7942 is described. Cells are lysed in modified sucrose, Triton X-100, EDTA, Tris buffer with phenol/chloroform. The cleared lysate is extracted further with phenol/chloroform and RNA is peleted by centrifugation through a 5.7 M CsCl cushion. High quality RNA can be prepared in three hours using this procedure.

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