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. 2006 Sep;13(9):849-51.
doi: 10.1038/nsmb1138. Epub 2006 Aug 20.

Perfect seed pairing is not a generally reliable predictor for miRNA-target interactions

Dominic Didiano  1 , Oliver Hobert
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Perfect seed pairing is not a generally reliable predictor for miRNA-target interactions

Dominic Didiano et al. Nat Struct Mol Biol. 2006 Sep.

Abstract

We use Caenorhabditis elegans to test proposed general rules for microRNA (miRNA)-target interactions. We show that G.U base pairing is tolerated in the 'seed' region of the lsy-6 miRNA interaction with its in vivo target cog-1, and that 6- to 8-base-pair perfect seed pairing is not a generally reliable predictor for an interaction of lsy-6 with a 3' untranslated region (UTR). Rather, lsy-6 can functionally interact with its target site only in specific 3' UTR contexts. Our findings illustrate the difficulty of establishing generalizable rules of miRNA-target interactions.

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Comment in

  • L(ou)sy miRNA targets?
    Rajewsky N. Rajewsky N. Nat Struct Mol Biol. 2006 Sep;13(9):754-5. doi: 10.1038/nsmb0906-754. Nat Struct Mol Biol. 2006. PMID: 16955093 No abstract available.

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