Stability of RNA isolated from macrophages depends on the removal of an RNA-degrading activity early in the extraction procedure
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- PMID: 1692714
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Stability of RNA isolated from macrophages depends on the removal of an RNA-degrading activity early in the extraction procedure
Biotechniques.
1990 Apr.
Erratum in
- Biotechniques 1990 Jul;9(1):55
Abstract
Ubiquitous RNases are the usual causes of RNA degradation on its isolation from mammalian cells. Using guanidine hydrochloride for the extraction of RNA from mouse peritoneal macrophages, we identify a major source of RNA-degrading activity, the stage of the extraction procedure at which this activity may be detected and show that its removal early in the extraction leads to a more dependable method for the recovery of high quality RNA.
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