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Comparative Study
. 2006 Sep;82(3):1043-50.
doi: 10.1016/j.athoracsur.2006.03.120.

Respiratory viral infection in obliterative airway disease after orthotopic tracheal transplantation

Elbert Kuo  1 , Ankit Bharat, Trudie Goers, Will Chapman, Le Yan, Tyler Street, Wei Lu, Michael Walter, Alexander Patterson, Thalachallour Mohanakumar
Affiliations
Comparative Study

Respiratory viral infection in obliterative airway disease after orthotopic tracheal transplantation

Elbert Kuo et al. Ann Thorac Surg. 2006 Sep.

Abstract

Background: The long-term survival after human lung transplantation is limited by bronchiolitis obliterans syndrome (BOS). Clinically, community-acquired respiratory viral infections have been correlated with an increased incidence of BOS. The goal of this study was to investigate the role of respiratory viral infections in chronic lung allograft rejection using the murine orthotopic tracheal transplantation model.

Methods: Eighty orthotopic tracheal transplants were performed using BALB/c and C57BL/6 mice. Recipient mice were infected intranasally with Sendai virus (SdV), a murine parainfluenza type I virus. Experiments altering the infectious dose, infection time, harvest time, allogeneic response, and viral response were performed. Tracheal allograft rejection was monitored using percent fibrosis and lamina propria to cartilage ratio measurements. Interferon-gamma ELISPOT analysis against irradiated donor (BALB/c) splenocytes was used as immunologic indicator of alloreactivity after transplantation.

Results: Sendai virus infection revealed a dose-dependent transient suppression of alloreactivity with a decrease in tracheal allograft fibrosis and frequency of alloreactive T cells at 30 days. This immunosuppression was reversed by day 60, leading to increased tracheal allograft fibrosis with a concomitant increase in the frequency of interferon-gamma producing alloreactive T cells. Pretransplant sensitization with donor antigens prevented the initial suppression of alloreactivity due to SdV infection. Furthermore, pretransplant immunization against SdV infection resulted in rapid clearing of the infection and reduced the immunopathology of rejection.

Conclusions: Respiratory viral infections can cause enhanced tracheal allograft rejection despite the initial phase of transient immunosuppression. Early treatment or vaccination against the respiratory infections may represent a viable intervention to reduce the risk of chronic rejection.

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Comment in

  • Invited commentary.
    van der Kaaij NP, Bogers AJ. van der Kaaij NP, et al. Ann Thorac Surg. 2006 Sep;82(3):1050-1. doi: 10.1016/j.athoracsur.2006.06.023. Ann Thorac Surg. 2006. PMID: 16928533 No abstract available.

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