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Review
. 2007 Jan;81(1):20-9.
doi: 10.1128/JVI.01358-06. Epub 2006 Aug 23.

A contemporary view of coronavirus transcription

Stanley G Sawicki  1 , Dorothea L Sawicki, Stuart G Siddell
Affiliations
Review

A contemporary view of coronavirus transcription

Stanley G Sawicki et al. J Virol. 2007 Jan.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Organization and expression of the MHV-A59 genome. The structural relationships of the MHV-A59 genome- and subgenome-length mRNAs are shown. The virus ORFs are depicted in teal (nsp1-nsp16 genes), blue (ns2, ns4a, ns4b, and ns5a genes), and green (S, M, E, N, and I structural protein genes). The ORFs are defined by the genomic sequence of MHV-A59 as published by Coley et al. (20). The open red box represents the common 59-leader sequence, and the barred circle represents the programmed (−1) frameshifting element. The translation products of the genome- and subgenome-length mRNAs are depicted, and the autoproteolytic processing of the ORF1a and ORF1a/ORF1b polyproteins into proteins nsp1 to nsp16 is shown. A number of confirmed and putative functional domains in the nsp proteins are also indicated. NeU, uridylate-specific endoribonuclease; PL1, papain-like protease 1; PL2, papain-like protease 2.
FIG. 2.
FIG. 2.
Model for coronavirus replication-transcription. The ORF1 of genomic RNA (red) is translated to produce pp1a and pp1ab, which assemble into an RTC (teal oval) that recognizes cis-acting elements at the 5′ and 3′ ends of the genome. This RTC copies the genome either continuously into genome-length template or discontinuously into the various subgenome-length minus-strand templates. The minus strands (blue) are used as templates for genomic and subgenomic mRNA synthesis. Only genomes are used as templates for minus-strand synthesis, i.e., replication. The RTCs engaging in plus-strand synthesis age and release their minus-strand templates, which are then degraded specifically.
See this image and copyright information in PMC

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