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Comparative Study
. 1990 May;28(5):944-8.
doi: 10.1128/jcm.28.5.944-948.1990.

Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae

R Rossau  1 , M Duhamel, E Van Dyck, P Piot, H Van Heuverswyn
Affiliations
Comparative Study

Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae

R Rossau et al. J Clin Microbiol. 1990 May.

Abstract

The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identified in contaminated cultures with the oligonucleotide probe. The 2.6-megadalton cryptic plasmid used as a probe for N. gonorrhoeae was shown to be less sensitive, detecting 179 of 181 N. gonorrhoeae isolates.

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References

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