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. 2006 Oct;2(10):529-30.
doi: 10.1038/nchembio816. Epub 2006 Aug 27.

Kinetic flux profiling of nitrogen assimilation in Escherichia coli

Jie Yuan  1 , William U Fowler, Elizabeth Kimball, Wenyun Lu, Joshua D Rabinowitz
Affiliations

Kinetic flux profiling of nitrogen assimilation in Escherichia coli

Jie Yuan et al. Nat Chem Biol. 2006 Oct.

Abstract

We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

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