Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2006 Sep 5;103(36):13265-6.
doi: 10.1073/pnas.0606106103. Epub 2006 Aug 28.

Proline to the rescue

Mark T Fisher  1
Affiliations
Comment

Proline to the rescue

Mark T Fisher. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

Conflict of interest statement: No conflicts declared.

Figures

Fig. 1.
Fig. 1.
Ignatova and Gierasch (2) have constructed the P39A CRABP–FlAsH and polyQ Htt53 CRABP–FlAsH model systems to monitor the kinetic progress of in vivo protein aggregation, thus allowing them to directly follow the generation of amorphous or fibrillar protein aggregation caused either by protein misfolding or amyloid formation (A, Upper and Lower, respectively). The aggregation was easily observed through the development of distinct fluorescent foci within the cell. By rapidly increasing the intracellular concentration of the naturally occurring osmolyte proline (B) using an up-regulation of the ProP (proline transporter), these investigators were able to monitor, for the first time, both the inhibition of aggregation and the shift in the dynamics on the real-time kinetics of disaggregation of amorphous and fibrillar aggregations in response to a substantial increase in intracellular proline concentrations (B). These experiments demonstrate a potentially easy methodology to rapidly control intracellular aggregation reactions simply by rapidly increasing the concentration of a particular intracellular osmolyte.
See this image and copyright information in PMC

Comment on

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nollen E. A. A., Garcia S. M., van Haaften G., Kim S., Chavez A., Morimoto R. I., Plasterk R. H. A. Proc. Natl. Acad. Sci. USA. 2004;101:6403–6408. - PMC - PubMed
    1. Ignatova Z., Gierasch L. M. Proc. Natl. Acad. Sci. USA. 2006;103:13357–13361. - PMC - PubMed
    1. Ignatova Z., Gierasch L. M. Proc. Natl. Acad. Sci. USA. 2004;101:523–528. - PMC - PubMed
    1. Yancey P. H., Clark M. E., Hand S. C., Bowlus R. D., Somero G. N. Science. 1982;217:1214–1222. - PubMed
    1. Bolen D. W., Baskakov I. V. J. Mol. Biol. 2000;310:955–963. - PubMed

LinkOut - more resources