Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Sep;50(9):3173-4.
doi: 10.1128/AAC.00427-06.

Intracellular penetration and activity of DX-619 in human polymorphonuclear leukocytes

Isabel García  1 , Sofía Ballesta, Concepción Murillo, Evelio J Perea, Alvaro Pascual
Affiliations

Intracellular penetration and activity of DX-619 in human polymorphonuclear leukocytes

Isabel García et al. Antimicrob Agents Chemother. 2006 Sep.

Abstract

The intracellular penetration and activity of DX-619 in human polymorphonuclear leukocytes have been evaluated. DX-619 reached intracellular concentrations 10 times higher than the extracellular concentrations reached. Uptake was rapid, reversible, nonsaturable, and affected by environmental temperature, some metabolic inhibitors, and a soluble membrane activator. DX-619 showed intracellular activity against Staphylococcus aureus.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
DX-619 uptake by human PMNs and efflux of PMN-associated DX-619 after removal of the extracellular drug (n = 4). The extracellular concentration was 2 μg/ml. Error bars indicate standard deviations.
FIG. 2.
FIG. 2.
Activities of DX-619 and ciprofloxacin against intracellular S. aureus in human PMNs (n = 4). Data are expressed as percentages of surviving bacteria after 3 h of incubation relative to controls without antimicrobial agents (means ± standard deviations [error bars]). *, P was <0.05 relative to ciprofloxacin.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Baltch, A. L., L. H. Bopp, R. P. Smith, P. B. Michelsen, and W. J. Ritz. 2005. Antibacterial activities of gemifloxacin, levofloxacin, gatifloxacin, moxifloxacin and erythromycin against intracellular Legionella pneumophila and Legionella micdadei in human monocytes. J. Antimicrob. Chemother. 56:104-109. - PubMed
    1. Bogdanovich, T., D. Esel, L. M. Kelly, B. Bozdogan, K. Credito, G. Lin, K. Smith, L. M. Ednie, D. B. Hoellman, and P. C. Appelbaum. 2005. Antistaphylococcal activity of DX-619, a new des-F(6)-quinolone, compared to those of other agents. Antimicrob. Agents Chemother. 49:3325-3333. - PMC - PubMed
    1. Fujikawa, K., M. Chiba, M. Tanaka, and K. Sato. 2005. In vitro antibacterial activity of DX-619, a novel des-fluoro(6) quinolone. Antimicrob. Agents Chemother. 49:3040-3045. - PMC - PubMed
    1. Fukuda, Y., K. Yanagihara, H. Ohno, Y. Higashiyama, Y. Miyazaki, K. Tsukamoto, Y. Hirakata, K. Tomono, Y. Mizuta, T. Tashiro, and S. Kohno. 2006. In vivo efficacies and pharmacokinetics of DX-619, a novel des-fluoro(6) quinolone, against Streptococcus pneumoniae in a mouse lung infection model. Antimicrob. Agents Chemother. 50:121-125. - PMC - PubMed
    1. García, I., A. Pascual, M. C. Guzmán, and E. J. Perea. 1992. Uptake and intracellular activity of sparfloxacin in human polymorphonuclear leukocytes and tissue culture cells. Antimicrob. Agents Chemother. 36:1053-1056. - PMC - PubMed

MeSH terms

LinkOut - more resources