Encapsulation and suspension of hydrophobic liquids via electro-hydrodynamics

Abstract

There are situations in which bioactive products of interest in biotechnology turn out to be hydrophobic. To reach high uniform levels of such products in water-based host fluids, such as those existing in many biological environments, one strategy consists on dividing the bioactive product into tiny micrometer (or sub-micrometer) pieces, since these are much more amenable of being uniformly dispersed and stabilized in the host fluid. On the other hand, if the bioactive product must act at specific locations, these micrometer pieces need to be hold in place, an objective that may be achieved by encapsulating them in mats of fibers. Here we demonstrate how these tasks may be accomplished by resorting to the generation and control of electrified coaxial jets of a hydrophilic liquid surrounding the hydrophobic liquid carrying the bioactive substance. When the process is carried out inside a dielectric liquid, double oil/water/oil and simple oil/water emulsions may be formed. On the other hand, when the process runs in air and a biopolymer is added to the hydrophilic liquid, then non woven mats of beaded nanofibers, encapsulating the bioactive product in the beads, are generated.