Immunohistochemical study of ependymal neoplasms: histological subtypes and glial and epithelial characteristics

Abstract

An immunohistochemical study on ependymal tumours was performed in order to determine what relationships exist between histological subtypes and epithelial or glial characteristics. Thirty-eight ependymal tumours were examined with antibodies to cytokeratin (CK), epithelial membrane antigen (EMA), transthyretin (TTR) and glial fibrillary acidic protein (GFAP) using the avidin-biotin-complex technique. They included 23 ependymomas, 13 anaplastic ependymomas, and 2 myxopapillary ependymomas. Only 3 of the 23 ependymomas were positive with EMA but 19 reacted with GFAP. None of them were positive with CK. Six of the 13 anaplastic ependymomas were positive with EMA, 3 with CK and 10 with GFAP. Five of the 6 anaplastic ependymomas which had epithelial marker proteins were either negative or weakly positive for GFAP. The present study demonstrates that most benign ependymomas exhibit GFAP positivity while the anaplastic ones tend to suppress their glial nature in favour of epithelial differentiation. However, ependymal tumours showed few characteristics of choroid plexus cells; only one of the examined cases was positive for TTR.