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. 2006 Nov;72(11):7406-9.
doi: 10.1128/AEM.01157-06. Epub 2006 Sep 1.

Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases

Mi-Hwa Lee  1 , Choong-Hwan Lee, Tae-Kwang Oh, Jae Kwang Song, Jung-Hoon Yoon
Affiliations

Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases

Mi-Hwa Lee et al. Appl Environ Microbiol. 2006 Nov.

Abstract

We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.

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Figures

FIG. 1.
FIG. 1.
Phylogenetic analysis of LipG and closely related proteins. Phylogenetic analysis was performed using the program MEGALIGN (DNASTAR, Madison, WI). Except for LipG, the protein sequences for previously identified families of bacterial lipolytic enzymes were retrieved from GenBank (http://www.ncbi.nlm.nih.gov). The units at the bottom of the tree indicate the number of substitution events.
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References

    1. Arpigny, J. L., and K. E. Jaeger. 1999. Bacterial lipolytic enzymes: classification and properties. Biochem. J. 343:177-183. - PMC - PubMed
    1. Brettar, I., R. Christen, and M. G. Hofle. 2003. Idiomarina balticasp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea. Int. J. Syst. Evol. Microbiol. 53:407-413. - PubMed
    1. Bugg, T. D. 2004. Diverse catalytic activities in the αβ-hydrolase family of enzymes: activation of H2O, HCN, H2O2, and O2. Bioorg. Chem. 32:367-375. - PubMed
    1. Bulow, L., and K. Mosbach. 1987. The expression in E. coli of a polymeric gene coding for an esterase mimic catalyzing the hydrolysis of p-nitrophenyl esters. FEBS Lett. 210:147-152. - PubMed
    1. Daniel, R. 2005. The metagenomics of soil. Nat. Rev. Microbiol. 3:470-478. - PubMed

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