[Isolation of human serum lipoproteins using a sedimentation method and the determination of their lipid composition]

Abstract

A method for separation of human blood serum lipoproteins into 3 fractions (low- and high-density lipoproteins, subfractions 2 and 3) is described in detail. The method may be used to rapidly obtain information on the status of lipid metabolism in humans and animals. The results of lipid composition analysis of individual lipoprotein fractions are presented. These data were obtained in a series of experiments and EC-1052 computer-processed with the use of a COM applied program pack. Basing on the results of investigation of individual fractions' lipid composition, the authors make a conclusion on the characteristic features of each fraction, which in future may be helpful in the diagnosis of pathologic process and in detection of lipid metabolism disorders in the body.