A modified Christensen's urea and CLSI broth microdilution method for testing susceptibilities of six Malassezia species to voriconazole, itraconazole, and ketoconazole
- PMID: 16954293
- PMCID: PMC1594707
- DOI: 10.1128/JCM.00989-06
A modified Christensen's urea and CLSI broth microdilution method for testing susceptibilities of six Malassezia species to voriconazole, itraconazole, and ketoconazole
Abstract
Two supplemented broths (Christensen's urea with 0.1% Tween 80 and 0.5% Tween 40 and RPMI 1640 with 1% glycerol, 1% peptone, 1.8% glucose, and 0.05% Tween 80) were evaluated to determine voriconazole, itraconazole, and ketoconazole MICs for 200 Malassezia sp. isolates. Malassezia globosa and M. restricta were the least susceptible species (MICs at which 90% of the isolates tested were inhibited, 1 to >or=8 microg/ml versus 0.25 to 1 microg/ml).
References
-
- Ahn, K. J., and H. R. Ashbee. 1996. Determination of minimum inhibitory concentrations of several azole antifungals for Malassezia furfur. Ann. Dermatol. 8:187-194.
-
- Clinical and Laboratory Standards Institute. 2006. Quality control minimal inhibitory concentration (MIC) limits for broth dilution and MIC interpretative breakpoints (M27-S2). Clinical and Laboratory Standards Institute, Wayne, Pa.
-
- Crespo, V., and V. Delgado. 2002. Malassezia species in skin diseases. Curr. Opin. Infect. Dis. 15:133-142. - PubMed
-
- Delescluse, J. 1990. Itraconazole in tinea versicolor: a review. J. Am. Acad. Dermatol. 23:551-554. - PubMed
-
- Espinel-Ingroff, A., K. Boyle, and D. Sheenan. 2001. In vitro antifungal activities of voriconazole and reference agents as determined by NCCLS methods: review of the literature. Mycopathologia 150:101-115. - PubMed
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