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. 2006 Sep;44(9):3443-7.
doi: 10.1128/JCM.01175-06.

Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2

Hatim T Allawi  1 , Haijing Li, Tamara Sander, Azamat Aslanukov, Victor I Lyamichev, Amondrea Blackman, Slava Elagin, Yi-Wei Tang
Affiliations

Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2

Hatim T Allawi et al. J Clin Microbiol. 2006 Sep.

Abstract

We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity of approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.

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Figures

FIG. 1.
FIG. 1.
Schematic representation of the Invader Plus method. Details of the reaction conditions are provided in the text.
See this image and copyright information in PMC

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