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Review
. 2007 Mar;81(5):2095-101.
doi: 10.1128/JVI.01422-06. Epub 2006 Sep 6.

Human cytomegalovirus tropism for endothelial cells: not all endothelial cells are created equal

Michael A Jarvis  1 , Jay A Nelson
Affiliations
Review

Human cytomegalovirus tropism for endothelial cells: not all endothelial cells are created equal

Michael A Jarvis et al. J Virol. 2007 Mar.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Immunofluorescence micrograph showing HCMV-infected AECs. AECs were infected with HCMV. At day 4 postinfection, cells were stained with antibodies directed against the major viral transcriptional activator expressed at immediate early times of infection, IE-2 (Cy5 [blue]), and the major virion glycoprotein expressed at early/late times of infection, gB (fluorescein isothiocyanate [green]).
FIG. 2.
FIG. 2.
HCMV inhibits IL-1 and tumor necrosis factor alpha (TNF-α) proinflammatory pathways. ECs (HUVECs) were infected with HCMV. At day 3 postinfection, cells were treated with proinflammatory cytokines (in this case, IL-1β). The effect of HCMV infection on cellular proinflammatory signaling pathways was then determined by an immunofluorescence analysis of the IL-1β/TNF-α-induced chemokine IL-8. Antibodies used were directed against gB (fluorescein isothiocyanate [green]) and cellular IL-8 (Texas Red [red]). The absence of IL-8 in HCMV (gB-positive) cells shows the profound inhibitory effect of HCMV on proinflammatory pathways.
FIG. 3.
FIG. 3.
Schematic showing roles of pUL128, pUL130, and pUL131A in EC tropism. The gH/gL glycoproteins form a disulfide-linked complex that is essential for viral entry and fusion. The gH/gL exists in two distinct forms, one composed of pUL128, pUL130, and pUL131A and the other composed of gO alone. The gH/gL/pUL128/pUL130/pUL131A complex is required for pH-dependent entry into ECs, whereas the gH/gL/gO complex is required for pH-independent entry into fibroblasts. The pUL128 and pUL130 proteins have been found in association with gH/gL. Although UL131A is required at early times of infection of ECs, the presence of pUL131A in the complex has not been definitively demonstrated. The gO protein is found in association with gH/gL and is required for replication in fibroblasts. However, the requirement of gO for entry into ECs has not been determined.
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