Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Sep;72(9):6325-30.
doi: 10.1128/AEM.03056-05.

Detection of Enterobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture

N R Mullane  1 , J Murray, D Drudy, N Prentice, P Whyte, P G Wall, A Parton, S Fanning
Affiliations

Detection of Enterobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture

N R Mullane et al. Appl Environ Microbiol. 2006 Sep.

Abstract

Enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. Contaminated infant milk formula (IMF) has been implicated in cases of E. sakazakii meningitis. Quick and sensitive methods to detect low-level contamination sporadically present in IMF preparations would positively contribute towards risk reduction across the infant formula food chain. Here we report on the development of a simple method, combining charged separation and growth on selective agar, to detect E. sakazakii in IMF. This protocol can reliably detect 1 to 5 CFU of E. sakazakii in 500 g of IMF in less than 24 h.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Schematic diagram showing the Pathatrix system. All relevant features are indicated with arrows. The temperature-controlled pot is indicated.
FIG. 2.
FIG. 2.
Pooling protocol approved by the American Organization of Analytical Chemistry for the simultaneous analysis of 500-g quantities of IMF. The dark box in the individual enrichment of samples represents an E. sakazakii-positive sample.
FIG. 3.
FIG. 3.
DFI agar plate showing mixed colony types containing E. sakazakii and S. enterica serotype Typhimurium DT104 (black).
See this image and copyright information in PMC

References

    1. Bar-Oz, B., A. Preminger, O. Peleg, C. Block, and I. Arad. 2001. Enterobacter sakazakii infection in the newborn. Acta Paediatr. 90:356-358. - PubMed
    1. Biering, G., S. Karlsson, N. C. Clark, K. E. Jonsdottir, P. Ludvigsson, and O. Steingrimsson. 1989. Three cases of neonatal meningitis caused by Enterobacter sakazakii in powdered milk. J. Clin. Microbiol. 27:2054-2056. - PMC - PubMed
    1. Blodget, R. 2001. Biological analytical manual, 8th ed. [Online.] http://www.cfsan.fda.gov/∼ebam/bam-a2.html. Accessed 20 March 2006.
    1. Daly, M., J. Buckley, E. Power, and S. Fanning. 2004. Evidence for a chromosomally located third integron in Salmonella enterica serovar Typhimurium DT104b. Antimicrob. Agents Chemother. 48:1350-1352. - PMC - PubMed
    1. Desinor, O. Y., J. L. Silva, and M. J. Menos. 2004. Neonatal sepsis and meningitis in Haiti. J. Trop. Pediatr. 50:48-50. - PubMed

Publication types

MeSH terms

LinkOut - more resources