Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis
Affiliations
- PMID: 16964802
Item in Clipboard
Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis
Int J Tuberc Lung Dis.
2006 Sep.
Abstract
We describe the simple adaptation of a standard fluorescent microscope for illumination using a 'Royal Blue' Luxeon light emitting diode (LED) and demonstrate that this form of illumination is suitable for the detection of auramine O stained Mycobacterium spp. The low cost, low power consumption, safety and reliability of LEDs makes them attractive alternatives to mercury vapour lamps.
Similar articles
-
The future looks bright: low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae.Trans R Soc Trop Med Hyg. 2008 Jun;102(6):520-1. doi: 10.1016/j.trstmh.2008.02.020. Epub 2008 Apr 10. Trans R Soc Trop Med Hyg. 2008. PMID: 18405928 Review.
-
Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum.Clin Infect Dis. 2008 Jul 15;47(2):203-7. doi: 10.1086/589248. Clin Infect Dis. 2008. PMID: 18532893
-
[Clinical evaluation of acid-fast smear examination with light emitting diode fluorescent microscopy].Kekkaku. 2009 Sep;84(9):627-9. Kekkaku. 2009. PMID: 19860265 Japanese.
-
Light-emitting diodes are better illumination sources for biological microscopy than conventional sources.Microsc Microanal. 2008 Jun;14(3):243-50. doi: 10.1017/S1431927608080288. Epub 2008 Mar 3. Microsc Microanal. 2008. PMID: 18312724
-
Light-emitting diodes in modern microscopy--from David to Goliath?Cytometry A. 2012 Mar;81(3):188-97. doi: 10.1002/cyto.a.22023. Epub 2012 Jan 30. Cytometry A. 2012. PMID: 22290727 Review.
Cited by
-
A disguised tuberculosis in oral buccal mucosa.Dent Res J (Isfahan). 2011 Summer;8(3):154-9. Dent Res J (Isfahan). 2011. PMID: 22013480 Free PMC article.
-
Comparison of LED and conventional fluorescence microscopy for detection of acid fast bacilli in a low-incidence setting.PLoS One. 2011;6(7):e22495. doi: 10.1371/journal.pone.0022495. Epub 2011 Jul 21. PLoS One. 2011. PMID: 21811622 Free PMC article.
-
New policies, new technologies: modelling the potential for improved smear microscopy services in Malawi.PLoS One. 2009 Nov 10;4(11):e7760. doi: 10.1371/journal.pone.0007760. PLoS One. 2009. PMID: 19901989 Free PMC article.
-
HIV-related incremental yield of bleach sputum concentration and fluorescence technique for the microscopic detection of tuberculosis.Eur J Clin Microbiol Infect Dis. 2008 Sep;27(9):849-55. doi: 10.1007/s10096-008-0516-4. Epub 2008 May 8. Eur J Clin Microbiol Infect Dis. 2008. PMID: 18463902
-
Retooling national TB control programmes (NTPs) with new diagnostics: the NTP perspective.PLoS One. 2010 Jul 19;5(7):e11649. doi: 10.1371/journal.pone.0011649. PLoS One. 2010. PMID: 20661463 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
