Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis
- PMID: 16964802
Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis
Abstract
We describe the simple adaptation of a standard fluorescent microscope for illumination using a 'Royal Blue' Luxeon light emitting diode (LED) and demonstrate that this form of illumination is suitable for the detection of auramine O stained Mycobacterium spp. The low cost, low power consumption, safety and reliability of LEDs makes them attractive alternatives to mercury vapour lamps.
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