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. 1990 Sep;10(9):4987-9.
doi: 10.1128/mcb.10.9.4987-4989.1990.

Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation

J Singer-Sam  1 , M Grant, J M LeBon, K Okuyama, V Chapman, M Monk, A D Riggs
Affiliations

Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation

J Singer-Sam et al. Mol Cell Biol. 1990 Sep.

Abstract

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

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References

    1. Science. 1989 Jan 6;243(4887):80-3 - PubMed
    1. Nature. 1979 Sep 27;281(5729):311-3 - PubMed
    1. Mol Cell Biol. 1988 Nov;8(11):4692-9 - PubMed
    1. Mol Cell Biol. 1986 Nov;6(11):4122-5 - PubMed
    1. Annu Rev Genet. 1988;22:199-233 - PubMed

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