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. 2006 Dec;72(12):7930-2.
doi: 10.1128/AEM.01040-06. Epub 2006 Sep 15.

A naturally occurring novel allele of Escherichia coli outer membrane protein A reduces sensitivity to bacteriophage

Michelle L Power  1 , Belinda C Ferrari, Jane Littlefield-Wyer, David M Gordon, Martin B Slade, Duncan A Veal
Affiliations

A naturally occurring novel allele of Escherichia coli outer membrane protein A reduces sensitivity to bacteriophage

Michelle L Power et al. Appl Environ Microbiol. 2006 Dec.

Abstract

A novel Escherichia coli outer membrane protein A (OmpA) was discovered through a proteomic investigation of cell surface proteins. DNA polymorphisms were localized to regions encoding the protein's surface-exposed loops which are known phage receptor sites. Bacteriophage sensitivity testing indicated an association between bacteriophage resistance and isolates having the novel ompA allele.

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Figures

FIG. 1.
FIG. 1.
Translation of the E. coli ompA1 and ompA2 alleles from three E. coli isolates H474 (human), TA024 (Tasmanian devil), and B194 (bird) and alignment to the E. coli and S. flexneri ompA sequences (GenBank accession no. ECOMPA and AY305875, respectively). Variations occur within the exposed loops of the protein (shaded gray). A single amino acid change was identified external to a loop region in isolate TA024 (shaded black).
FIG. 2.
FIG. 2.
Frequency of Escherichia coli strains carrying the ompA2 allele with respect to source of strain and ECOR group membership of the strains.
See this image and copyright information in PMC

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