Persistence of antibodies in blood and body fluids in decaying fox carcasses, as exemplified by antibodies against Microsporum canis

Abstract

To assist in evaluating serological test results from dead animals, 10 silver foxes (Vulpes vulpes) and 10 blue foxes (Alopex lagopus), 6 of each species previously vaccinated against and all challenged with Microsporum canis, were blood sampled and euthanased. Fox carcasses were stored at +10 degrees C, and autopsy was performed on Days 0, 2, 4, 7, and 11 post mortem during which samples from blood and/or body fluid from the thoracic cavity were collected. Antibodies against M. canis were measured in an enzyme-linked immunosorbent assay (ELISA) as absorbance values (optical density; OD). To assess the degradation of antibodies, the ratio between post mortem and ante mortem absorbance was calculated. The mean absorbance from samples collected during autopsy was generally lower than from samples from live animals. In blood samples, this difference increased significantly with time (P = 0.04), while in body fluid samples the difference decreased (not significant; P = 0.18). We suggest that a positive serological result from testing blood or body fluid of a dead animal may be regarded as valuable, although specific prevalences obtained by screening populations based on this type of material may represent an under-estimation of the true antibody prevalence. Negative serological test results based on material from carcasses may be less conclusive, taken into account the general degradation processes in decaying carcasses, also involving immunoglobulin proteins.

Figure 1

Linear regression plot for the relationship between the post mortem blood/ante mortem blood specific antibody ratio and time. Antibodies measured as absorbance (OD₄₅₀). The lines surrounding the linear regression line indicate 95% confidence and prediction intervals.

Figure 2

Linear regression plot for the relationship between the post mortem body fluid/ante mortem blood specific antibody ratio and time. Antibodies measured as absorbance (OD₄₅₀). The lines surrounding the linear regression line indicate 95% confidence and prediction intervals.