Predicted structure of rabbit N-terminal, calcitonin and katacalcin peptides

Abstract

Poly A rich RNA was extracted from rabbit thyroid and cDNA obtained by the action of reverse transcriptase. The cDNA was used to construct a library in lambda GT 11. Screening of the library with a radio-labelled probe specific for human calcitonin allowed the isolation of a clone containing an open reading frame with a high homology with human and murine exon 4 of calcitonin/calcitonin gene-related peptide gene. This sequence codes for a typical calcitonin precursor. We deduced the amino acid sequence of rabbit N-terminal peptide, calcitonin and katacalcin.