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. 2007 Mar;81(6):3009-11.
doi: 10.1128/JVI.01663-06. Epub 2006 Sep 27.

Infectious RNA isolated from the spinal cords of mice chronically infected with Theiler's murine encephalomyelitis virus

Jane E Libbey  1 , Ikuo Tsunoda, J Lindsay Whitton, Robert S Fujinami
Affiliations

Infectious RNA isolated from the spinal cords of mice chronically infected with Theiler's murine encephalomyelitis virus

Jane E Libbey et al. J Virol. 2007 Mar.

Abstract

The DA strain of Theiler's murine encephalomyelitis virus (TMEV) persistently infects cells of the spinal cord during the chronic phase of infection. Although in situ hybridization and reverse transcription-PCR have demonstrated the presence of viral RNA in the spinal cord, it has not been determined whether this RNA is infectious and, if so, how many PFU equivalents of virus the RNA can yield. In this study, we demonstrated that TMEV RNA isolated from the spinal cords of chronically infected mice is infectious and that there is at least 30-fold more infectious RNA than infectious virus in the spinal cords of these mice.

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