Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Nov;44(11):4242-5.
doi: 10.1128/JCM.01558-06. Epub 2006 Sep 27.

Integrons containing the VIM-2 metallo-beta-lactamase gene among imipenem-resistant Pseudomonas aeruginosa strains from different Chinese hospitals

Yun-Song Yu  1 , Ting-Ting Qu, Jian-Ying Zhou, Jie Wang, Hong-Yang Li, Timothy R Walsh
Affiliations

Integrons containing the VIM-2 metallo-beta-lactamase gene among imipenem-resistant Pseudomonas aeruginosa strains from different Chinese hospitals

Yun-Song Yu et al. J Clin Microbiol. 2006 Nov.

Abstract

A total of 140 nonrepetitive strains of imipenem-resistant Pseudomonas aeruginosa were isolated from five different Chinese hospitals. Fourteen isolates were confirmed to contain the VIM-2 metallo-beta-lactamase gene. Twelve isolates harbored two kinds of class 1 integron, containing both VIM-2- and aminoglycoside-resistant genes.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Locations of the five cities in China involved in this study.
FIG. 2.
FIG. 2.
Schematic integron structures (not to scale) of integrons in isolates P. aeruginosa B2, B3, B28, H19, H26, H54, and G4 (A) and P. aeruginosa S5, S9, S10, S11, and S12 (B). These integrons had approximate sizes of 2.5 and 3 kb, respectively. The open reading frames are shown as arrows, which also indicate their transcriptional orientations.
FIG. 3.
FIG. 3.
Southern hybridization analysis of the XbaI-digested chromosomal DNA fragments from the 14 strains containing the blaVIM-2 gene. PFGE patterns (A) are shown. M, lambda molecular weight ladder. (B) Results from Southern hybridization using a blaVIM-2 DNA probe.
See this image and copyright information in PMC

References

    1. Aubron, C., L. Poirel, N. Fortineau, P. Nicolas, L. Collet, and P. Nordmann. 2005. Nosocomial spread of Pseudomonas aeruginosa isolates expressing the metallo-beta-lactamase VIM-2 in a hematology unit of a French hospital. Microb. Drug Resist. 11:254-259. - PubMed
    1. Bachmann, B. J., and K. B. Low. 1980. Linkage map of Escherichia coli K-12, edition 6. Microbiol. Rev. 44:1-56. - PMC - PubMed
    1. Castanheira, M., M. A. Toleman, R. N. Jones, F. J. Schmidt, and T. R. Walsh. 2004. Molecular characterization of a beta-lactamase gene, blaGIM-1, encoding a new subclass of metallo-beta-lactamase. Antimicrob. Agents Chemother. 48:4654-4661. - PMC - PubMed
    1. Clinical and Laboratory Standards Institute. 2006. Performance standards for antimicrobial susceptibility testing: 16th informational supplement. CLSI document M100-S16. Clinical and Laboratory Standards Institute, Wayne, Pa.
    1. Fluit, A. C., and F. J. Schmitz. 1999. Class 1 integrons, gene cassettes, mobility, and epidemiology. Eur. J. Clin. Microbiol. Infect. Dis. 18:761-770. - PubMed

Publication types

MeSH terms

LinkOut - more resources