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. 2006 Oct;50(10):3485-7.
doi: 10.1128/AAC.00363-06.

SME-3, a novel member of the Serratia marcescens SME family of carbapenem-hydrolyzing beta-lactamases

Anne Marie Queenan  1 , Wenchi Shang, Paul Schreckenberger, Karen Lolans, Karen Bush, John Quinn
Affiliations

SME-3, a novel member of the Serratia marcescens SME family of carbapenem-hydrolyzing beta-lactamases

Anne Marie Queenan et al. Antimicrob Agents Chemother. 2006 Oct.

Abstract

Imipenem-resistant Serratia marcescens isolates were cultured from a lung transplant patient given multiple antibiotics over several months. The strains expressed SME-3, a beta-lactamase of the rare SME carbapenem-hydrolyzing family. SME-3 differed from SME-1 by a single amino acid substitution of tyrosine for histidine at position 105, but the two beta-lactamases displayed similar hydrolytic profiles.

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Figures

FIG. 1.
FIG. 1.
PFGE of SpeI-digested DNA from S. marcescens isolates. Lanes 1 to 4, imipenem-resistant strains with characterized SME β-lactamases (9); lane 1, S6; lane 2, OC4124; lane 3, OC4126; lane 4, OC4176; lanes 5 and 6, independently isolated imipenem-resistant strains from lung transplant patient; lane 7, imipenem-susceptible isolate from the same patient.
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