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. 1975;2(3):273-80.
doi: 10.3109/07435807509053854.

Estrogen binding protein of rat liver

P Viladiu, C Delgado, J Pensky, O H Pearson

Estrogen binding protein of rat liver

P Viladiu et al. Endocr Res Commun. 1975.

Abstract

An estrogen binding protein for estradiol-17beta is present in the liver cytosol of female intact and one day oophorectomized rats. The dissociation constant reveals high affinity binding (Kd: 0.69 +/- 0.14 times 10(-10) M). Quantitation of EBP using a dextran-coated charcoal method shows that this specific macromolecular binding is much less than in the rat uterus, but similar to that in DMBA-induced mammary tumors. Sucrose density gradient analysis shows sedimentation at 8-9 S and 4-5 S when compared to bovine serum albumin.

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