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. 2006 Sep 21;12(35):5668-73.
doi: 10.3748/wjg.v12.i35.5668.

Mechanical behavior of colonic anastomosis in experimental settings as a measure of wound repair and tissue integrity

Konstantinos A Ekmektzoglou  1 , Georgios C Zografos, Stavros K Kourkoulis, Ismene A Dontas, Panagiotis K Giannopoulos, Katerina A Marinou, Maria V Poulakou, Despina N Perrea
Affiliations

Mechanical behavior of colonic anastomosis in experimental settings as a measure of wound repair and tissue integrity

Konstantinos A Ekmektzoglou et al. World J Gastroenterol. .

Abstract

Aim: To determine the mechanical properties of anastomotic colonic tissue in experimental settings and therefore give a measure of wound healing.

Methods: Thirty-six male Wistar rats were used as experimental models of anastomotic tissue integrity. On the 5th post-operative day, the tensile strength was measured by application of an axial force, providing a quantitative measure of anastomotic dehiscence and leakage.

Results: Diagrams of the load as a function of the time [P = P (t)] and of the displacement also as a function of time [Delta s = Delta s (t)] were recorded for each test, permitting the design of the load versus the displacement diagram and thus providing significant data about the critical values of anastomotic failure. Quantitative data were obtained concerning the anastomotic strength of both control specimens (healthy rats), as well as specimens from non-healthy rats for comparison.

Conclusion: This experimental model provides an excellent method of measuring anastomotic strength. Despite the relative small number of specimens used, this method provides an accurate way of measuring wound repair. More experimental measurements need to be performed to correlate emerging tensile strength values to anastomotic failure.

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Figures

Figure 1
Figure 1
Typical forces applied on a standard specimen.
Figure 2
Figure 2
The application of hydraulic pressure creating a two-dimensional stress field.
Figure 3
Figure 3
The gripping system consisting of a pair of light metallic pins. The grooves at which the intestine is gripped are indicated by the arrows.
Figure 4
Figure 4
Load versus time and displacement diagrams for characteristic tests. A: Load versus displacement for a typical test of the preliminary series; B: Load versus time using intact specimens from healthy rats; C: Load versus time using specimens from healthy rats after colonic anastomosis; D: Load versus time using specimens from non-healthy rats after colonic anastomosis.
See this image and copyright information in PMC

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