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. 1990 Oct 30;172(2):511-6.
doi: 10.1016/0006-291x(90)90702-o.

A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

K Muraiso  1 , M Hijikata, S Ohkoshi, M J Cho, M Kikuchi, N Kato, K Shimotohno
Affiliations

A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

K Muraiso et al. Biochem Biophys Res Commun. .

Abstract

A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.

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