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. 1990 Nov 11;18(21):6347-51.
doi: 10.1093/nar/18.21.6347.

B2 RNA and 7SK RNA, RNA polymerase III transcripts, have a cap-like structure at their 5' end

G P Shumyatsky  1 , S V Tillib, D A Kramerov
Affiliations
Free PMC article

B2 RNA and 7SK RNA, RNA polymerase III transcripts, have a cap-like structure at their 5' end

G P Shumyatsky et al. Nucleic Acids Res. .
Free PMC article

Abstract

We found that hydrolysates of poly(A)+ RNA from Ehrlich ascites carcinoma cells which were transcribed by RNA polymerase III contained an unusual component designated as X. It was part of B2 RNA representing a transcript of B2 retroposon, typical of rodents. The component X possesses a cap-like structure, xppp5'G, where x has a non-nucleotide structure. About half of all B2 RNAs contained this group at the 5' end. Previously, Epstein et al. (1) detected a similar structure at the 5' end of small nuclear U6 RNA. Later, Singh and Reddy (2) showed methyl to be the blocking group in the component x of U6 RNA. Besides B2 RNA, we found 5' ends containing methyl groups in 7SK RNA.

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