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. 1990;54(1-2):6-9.
doi: 10.1159/000132944.

Analysis of the structure and variability of nucleolar organizer regions of Salmo trutta by C-, Ag-, and restriction endonuclease banding

L Sánchez  1 , P Martínez, A Viñas, C Bouza
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Analysis of the structure and variability of nucleolar organizer regions of Salmo trutta by C-, Ag-, and restriction endonuclease banding

L Sánchez et al. Cytogenet Cell Genet. 1990.

Abstract

Nucleolar organizer regions (NORs) of brown trout were investigated using C-, Ag-, and restriction endonuclease banding. The presence of constitutive heterochromatin was confirmed by C-banding. Giemsa-staining, C-banding, and Ag-banding revealed great variability in the size of the short arm of the NOR-bearing chromosome. This size variation was due in some cases to NOR duplication. Restriction endonuclease digestion induced a specific banding pattern for AluI, DdeI, HaeIII, MboI, and HinfI, indicating some features about the sequence composition of the NOR-associated heterochromatin.

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