doi: 10.1016/0014-5793(90)81327-k.
Blockage of AMV reverse transcriptase by antisense oligodeoxynucleotides
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- PMID: 1701402
- DOI: 10.1016/0014-5793(90)81327-k
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Blockage of AMV reverse transcriptase by antisense oligodeoxynucleotides
FEBS Lett.
.
Free article
Abstract
Synthetic oligodeoxynucleotides, either unmodified or linked to an intercalating agent, have been used to prevent cDNA elongation by the AMV reverse transcriptase. Oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA part bound to the antisense oligonucleotide by the RNase-H activity associated with the retroviral polymerase.
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