N-linked oligosaccharides during human renal organogenesis

Abstract

The structure of fucosylated derivatives of N-acetyl lactosamine expressed during human renal development has been studied by the combined use of monoclonal antibodies and glycosyl hydrolases. LeX is expressed on the ureteric bud ampulla carried N-linked on a glycoprotein. It is also present in the proximal tubule as a ganglioside. H-Type II substance is expressed on the endothelium of the developing renal vasculature. It is also carried N-linked to a glycoprotein. These regulated changes in the cell surface carbohydrate expression are important in understanding cell interactions and receptor systems during renal development.