Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Sep;12(3):536-43.
doi: 10.1128/iai.12.3.536-543.1975.

Affinity chromatography purification of Clostridium perfringens enterotoxin

V N Scott, C L Duncan

Affinity chromatography purification of Clostridium perfringens enterotoxin

V N Scott et al. Infect Immun. 1975 Sep.

Abstract

Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Cell extracts containing enterotoxin or partially purified toxin preparations were applied to the column and nonspecifically-bound protein was eluted. NaOH was used to elute specifically bound toxin. The purity of enterotoxin purified by Sephadex G-100 chromatography followed by affinity chromatography appears similar to toxin highly purified by conventional means. The procedure can be used successfully for the rapid (less than 2 h) purification of small amounts of enterotoxin.

PubMed Disclaimer

References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Clin Chim Acta. 1975 Jan 20;58(2):177-80 - PubMed
    1. J Mol Biol. 1974 Aug 5;87(2):317-27 - PubMed
    1. Appl Microbiol. 1968 Jan;16(1):82-9 - PubMed
    1. J Bacteriol. 1969 Oct;100(1):86-94 - PubMed

Publication types

LinkOut - more resources