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. 2006 Dec;44(12):4550-2.
doi: 10.1128/JCM.01542-06. Epub 2006 Oct 4.

Monitoring of stool microbiota in subjects with diarrhea indicates distortions in composition

Volker Mai  1 , Christopher R Braden, Jill Heckendorf, Baiba Pironis, Jon Mark Hirshon
Affiliations

Monitoring of stool microbiota in subjects with diarrhea indicates distortions in composition

Volker Mai et al. J Clin Microbiol. 2006 Dec.

Abstract

We utilized denaturing gradient gel electrophoresis profiling to survey stool microbiota in 175 persons with diarrhea and 113 asymptomatic persons in a diarrhea surveillance study. Compared with healthy controls, the microbiota profiles in diarrhea cases more frequently exhibited decreased diversity and strong bands indicating the overgrowth of selected bacteria or bacterial groups.

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Figures

FIG. 1.
FIG. 1.
DGGE gel showing representative fecal microbiota profiles of diarrhea patients (P) and controls (C). S, standard. Strong bands (lanes 2 and 6) are circled. Strong bands on the bottom of the gradient (lanes 5, 7, and 8) are not considered strong bands, as such bands frequently contain multiple molecular species that do not separate under the DGGE conditions used. Distorted DGGE profiles (at most eight detectable bands) are indicated by arrows (lanes 6 and 9). Lanes 7 and 8 show the DGGE profile from the stool of the same control subject for which DNA extraction and PCR amplification were done on different days indicating negligible experimental variation.
See this image and copyright information in PMC

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