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. 2006 Oct;44(10):3784-6.
doi: 10.1128/JCM.01373-06.

Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit

Shoko Okitsu-Negishi  1 , Michio Okame, Yuko Shimizu, Tung Gia Phan, Takeshi Tomaru, Shigenori Kamijo, Takashi Sato, Fumihiro Yagyu, Werner E G Müller, Hiroshi Ushijima
Affiliations

Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit

Shoko Okitsu-Negishi et al. J Clin Microbiol. 2006 Oct.

Abstract

The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.

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