Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2006 Oct;44(10):3804-7.
doi: 10.1128/JCM.00395-06.

Comparison of PCR-based methods for typing Staphylococcus aureus isolates

Artur Sabat  1 , Natalia Malachowa, Jacek Miedzobrodzki, Waleria Hryniewicz
Affiliations
Comparative Study

Comparison of PCR-based methods for typing Staphylococcus aureus isolates

Artur Sabat et al. J Clin Microbiol. 2006 Oct.

Abstract

In this study, we compared the potentials of (i) a multiplex PCR-based multilocus variable-number tandem repeat (VNTR) assay; (ii) a triplex PCR coamplifying fragments of spa, coa, and the hypervariable region adjacent to the mecA gene; (iii) restriction profile analysis of the STAR repetitive element; (iv) randomly amplified polymorphic DNA analysis; (v) inter-IS256 PCR; and (vi) rep-MP3 PCR. Multilocus VNTR typing and triplex PCR (coa, spa, and hypervariable region) approaches showed excellent reproducibility and high discriminatory power; however, only multilocus VNTR typing could distinguish all pulsed-field gel electrophoresis and spa types. Multilocus VNTR typing appears to be the most useful PCR-based method for the rapid genotyping of Staphylococcus aureus strains.

PubMed Disclaimer

References

    1. Chiou, C. S., H. L. Wei, and L. C. Yang. 2000. Comparison of pulsed-field gel electrophoresis and coagulase gene restriction profile analysis techniques in the molecular typing of Staphylococcus aureus. J. Clin. Microbiol. 38:2186-2190. - PMC - PubMed
    1. Chung, M., H. de Lencastre, P. Matthews, A. Tomasz, I. Adamsson, M. Aires de Sousa, T. Camou, C. Cocuzza, A. Corso, I. Couto, A. Dominguez, M. Gniadkowski, R. Goering, A. Gomes, K. Kikuchi, A. Marchese, R. Mato, O. Melter, D. Oliveira, R. Palacio, R. Sa-Leão, I. Santos Sanches, J. Song, P. T. Tassios, and P. Villari. 2000. Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis: comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains. Microb. Drug Resist. 6:189-198. - PubMed
    1. Deplano, A., A. Schuermans, J. Van Eldere, W. Witte, H. Meugnier, J. Etienne, H. Grundmann, D. Jonas, G. T. Noordhoek, J. Dijkstra, A. van Belkum, W. van Leeuwen, P. T. Tassios, N. J. Legakis, A. van der Zee, A. Bergmans, D. S. Blanc, F. C. Tenover, B. C. Cookson, G. O'Neil, M. J. Struelens, and the European Study Group on Epidemiological Markers of the ESCMID. 2000. Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive-element PCR analysis. J. Clin. Microbiol. 38:3527-3533. - PMC - PubMed
    1. Deplano, A., M. Vaneechoutte, G. Verschraegen, and M. J. Struelens. 1997. Typing of Staphylococcus aureus and Staphylococcus epidermidis strains by PCR analysis of inter-IS256 spacer length polymorphisms. J. Clin. Microbiol. 35:2580-2587. - PMC - PubMed
    1. Enright, M. C., N. P. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015. - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources