Caution on interpretation of legionella results obtained using real-time PCR for environmental water samples

Comment

Hsiu-Yun Shih et al. Appl Environ Microbiol. 2006 Oct.

. 2006 Oct;72(10):6859.

doi: 10.1128/AEM.00968-06.

No abstract available

Comment on

Quantitative real-time Legionella PCR for environmental water samples: data interpretation.
Joly P, Falconnet PA, André J, Weill N, Reyrolle M, Vandenesch F, Maurin M, Etienne J, Jarraud S. Joly P, et al. Appl Environ Microbiol. 2006 Apr;72(4):2801-8. doi: 10.1128/AEM.72.4.2801-2808.2006. Appl Environ Microbiol. 2006. PMID: 16597985 Free PMC article.

1. Eej, A. K., M. H. Mahbubani, and R. M. Atlas. 1991. Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods. Appl. Environ. Microbiol. 57:597-600. - PMC - PubMed
1. Joly, P., P.-A. Falconnet, J. André, N. Weill, M. Reyrolle, F. Vandenesch, M. Maurin, J. Etienne, and S. Jarraud. 2006. Quantitative real-time Legionella PCR for environmental water samples: data interpretation. Appl. Environ. Microbiol. 72:2801-2808. - PMC - PubMed
1. Wellinghausen, N., C. Frost, and R. Marre. 2001. Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR. Appl. Environ. Microbiol. 67:3985-3993. - PMC - PubMed
1. Wilson, I. G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63:3741-3751. - PMC - PubMed
1. Yamamoto, H., Y. Hashimoto, and T. Ezaki. 1993. Comparison of detection methods for Legionella species in environmental water by colony isolation, fluorescent antibody staining, and polymerase chain reaction. Microbiol. Immunol. 37:617-622. - PubMed