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Review
. 2007 Jul;33(1):51-6.
doi: 10.1007/s00726-006-0410-2. Epub 2006 Oct 6.

Detecting oxidative post-translational modifications in proteins

E Gianazza  1 , J Crawford, I Miller
Affiliations
Review

Detecting oxidative post-translational modifications in proteins

E Gianazza et al. Amino Acids. 2007 Jul.

Abstract

Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids (Cys, Tyr, Met, Trp, Lys).

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