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. 1990 Nov 30;96(1):101-5.
doi: 10.1016/0378-1119(90)90347-t.

Cloning of a promoter used by sigma H RNA polymerase in Bacillus subtilis

H L Carter 3rd  1 , K M Tatti, C P Moran Jr
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Cloning of a promoter used by sigma H RNA polymerase in Bacillus subtilis

H L Carter 3rd et al. Gene. .

Abstract

The secondary RNA polymerase sigma factor sigma H is essential for endospore development in Bacillus subtilis. However, only a few promoters that are used by RNA polymerase containing sigma H (E sigma H) have been identified. We used in vitro transcription of random cloned fragments of B. subtilis chromosomal DNA to identify a promoter that is used by E sigma H. This promoter is active before the onset of sporulation.

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