Newly identified U4/U6 snRNP-binding proteins by serum autoantibodies from a patient with systemic sclerosis
- PMID: 1702805
Newly identified U4/U6 snRNP-binding proteins by serum autoantibodies from a patient with systemic sclerosis
Abstract
We found serum autoantibodies directed against the proteins binding exclusively to U4/U6 of Sm small nuclear ribonucleoprotein particle (snRNP) in serum from a patient (MaS) with systemic sclerosis. Their specificity, called anti-MaS, is distinct from that of known antibodies against U snRNP. The U4 and U6 small nuclear RNA from a 32P-labeled HeLa cell extract and five proteins with Mr 150,000, 120,000, 80,000, 36,000, and 34,000, in addition to Sm core proteins (B, B', D, E, F, and G) from an [35S] methionine-labeled extract, were immunoprecipitated by anti-MaS in isotonic solution. However, the Sm core proteins and U4 and U6 small nuclear RNA were separated from the protein-A-Sepharose facilitated MaS immunoprecipitate by incubation in a solution containing 500 mM NaCl. In immunoblots, anti-MaS antibodies reacted with one protein of Mr 150,000 from a HeLa cell nuclear extract that was fractionated by SDS-PAGE and transferred to a nitrocellulose sheet. The monospecific immunoaffinity purified antibody eluted from the immunoblot band immunoprecipitated U4 and U6 small nuclear RNA and reblotted the protein with Mr 150,000. These data indicate that anti-MaS antibodies recognize at least one antigenic protein that binds exclusively to the U4/U6 snRNP.
Similar articles
-
Characterization of autoantibodies that recognize U4/U6 small ribonucleoprotein particles in serum from a patient with primary Sjögren's syndrome.J Biol Chem. 1992 Aug 15;267(23):16412-6. J Biol Chem. 1992. PMID: 1386602
-
Further characterization and subcellular localization of Sm and U1 ribonucleoprotein antigens.Eur J Immunol. 1985 Oct;15(10):992-7. doi: 10.1002/eji.1830151006. Eur J Immunol. 1985. PMID: 2414112
-
U4 and U6 small nuclear ribonucleoprotein particles. 1. Fractionation and characterization of snRNPs containing U4 and U6 RNAs.Cytobios. 1988;53(214-215):143-51. Cytobios. 1988. PMID: 2968224
-
Monoclonal autoantibody recognizing a unique set of small nuclear ribonucleoprotein complexes.J Immunol. 1988 Jan 1;140(1):69-77. J Immunol. 1988. PMID: 2447172
-
Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal antibodies.J Immunol. 1989 Oct 15;143(8):2560-6. J Immunol. 1989. PMID: 2477448
Cited by
-
Roles of U4 and U6 snRNAs in the assembly of splicing complexes.EMBO J. 1992 Jan;11(1):335-43. doi: 10.1002/j.1460-2075.1992.tb05056.x. EMBO J. 1992. PMID: 1740112 Free PMC article.
-
Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs.Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6781-5. doi: 10.1073/pnas.90.14.6781. Proc Natl Acad Sci U S A. 1993. PMID: 8341699 Free PMC article.
-
Association of phosphorylated serine/arginine (SR) splicing factors with the U1-small ribonucleoprotein (snRNP) autoantigen complex accompanies apoptotic cell death.J Exp Med. 1998 Feb 16;187(4):547-60. doi: 10.1084/jem.187.4.547. J Exp Med. 1998. PMID: 9463405 Free PMC article.
-
Anti-U11/U12 RNP antibodies in systemic sclerosis: a new serologic marker associated with pulmonary fibrosis.Arthritis Rheum. 2009 Jul 15;61(7):958-65. doi: 10.1002/art.24586. Arthritis Rheum. 2009. PMID: 19565553 Free PMC article.
-
A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction.EMBO J. 1994 Jul 15;13(14):3356-67. doi: 10.1002/j.1460-2075.1994.tb06638.x. EMBO J. 1994. PMID: 8045264 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical