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. 1991 Jan;51(1):275-8.
doi: 10.1097/00007890-199101000-00048.

Effect of the immunosuppressant FK506 on glucose-induced insulin secretion from adult rat islets of Langerhans

P B Carroll  1 , A C Boschero, M Y Li, A G Tzakis, T E Starzl, I Atwater
Affiliations

Effect of the immunosuppressant FK506 on glucose-induced insulin secretion from adult rat islets of Langerhans

P B Carroll et al. Transplantation. 1991 Jan.
No abstract available

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Figures

Figure 1
Figure 1
The acute effect of FK506 on 16.7 mM glucose–induced insulin release. In this and subsequent figures insulin release was measured at the end of a 90-min incubation in all conditions. Values are expressed as the mean ± SEM from 36 batches of islets. In this and subsequent figures, 100% secretion corresponds to insulin measured in 16.7 mM glucose after subtraction of basal release. Basal (2.8 mM glucose induced) insulin release was 650±5.8 pg/islet/90 min and stimulated (16.7 mM glucose induced) release was 2208±20.9 pg/islet/90 min (P<0.01). A concentration of 100 ng/ml FK506 produced an average inhibition of 35% (P<0.05). This concentration was inhibitory in every experiment. In one experiment, a concentration of 10 ng/ml was also significantly inhibitory.
Figure 2
Figure 2
The acute effect of CsA on glucose induced insulin release. Values are expressed as the mean ± SEM from 12 batches of islets. Basal (2.8 mM glucose induced) insulin release was 229±0.93 pg/islet/90 min and stimulated (16.7 mM glucose induced) was 1556±10.2 pg/islet/90 min (P<0.01). CsA at concentrations of 1 and 10 μg/ml produced about a 30% inhibition of stimulated insulin release (P<0.05).
Figure 3
Figure 3
The combined effect of CsA and FK506 on glucose-induced insulin release. Results are expressed as the mean ± SEM from 12 batches of islets. Basal (2.8 mM glucose–induced) insulin release was 268±8.5 pg/islet/90 min, and stimulated (16.7 mM glucose induced) release was 1288±11.4 pg/islet/90 min (P<0.01). CsA produced a 40% and 50% reduction of glucose-induced insulin release at 1 and 10 μg/ml, respectively (P<0.05). The effects of CsA at either concentration and FK506 (1 ng/ml) were not additive to inhibit insulin release further.
Figure 4
Figure 4
Effect of 24-hr incubation with immune suppressant drugs on glucose induced insulin release. After 24-hr culture in the presence of CsA or FK506 in the concentrations shown, the islets were washed 5× then preincubated in 5.6 mM glucose containing buffer without drug. The medium was removed and insulin release was measured at the end of a 90-min incubation in all conditions. Values are expressed as the mean ± SEM from 12 batches of islets. Basal (5.6 mM glucose–induced) insulin release was 236±3.4 pg/islet/90 min and stimulated (16.7 mM glucose–induced) release was 1020±6.9 pg/islet/90 min (P<0.01). Previous exposure to CsA produced a 33% inhibition (P<0.05) of glucose-induced insulin release. FK506 did not produce a significant inhibition.
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References

    1. White DTG, editor. Cyclosporin A. New York: Elsevier; 1982.
    1. Yale JF, Grose M, Seemayer TA, Marliss EB. Immunological and metabolic concomitants of cyclosporin prevention of diabetes in BB rats. Diabetes. 1987;36:749. - PubMed
    1. Stiller CR, Dupre J, Gent M, et al. Effects of cyclosporine immunosuppression in insulin-dependent diabetes mellitus of recent onset. Science. 1984;223:1362. - PubMed
    1. Helmchen U, Schmidt WE, Siegel EG, Creutzfeldt W. Morphological and functional changes of pancreatic B-cells in cyclosporin A–treated rats. Diabetologia. 1984;27:416. - PubMed
    1. Andersson A, Borg H, Hallberg A, Hellerstrom C, Sandler S, Schnell A. Long-term effects of cyclosporin A on cultured mouse pancreatic islets. Diabetologia. 1984;27:66. - PubMed

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