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. 1991 Jan 21;47(2):193-201.
doi: 10.1002/ijc.2910470206.

Monoclonal antibodies to glutathione S-transferase pi-immunohistochemical analysis of human tissues and cancers

R R Kantor  1 , S L Giardina, A Bartolazzi, A J Townsend, C E Myers, K H Cowan, D L Longo, P G Natali
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Monoclonal antibodies to glutathione S-transferase pi-immunohistochemical analysis of human tissues and cancers

R R Kantor et al. Int J Cancer. .

Abstract

Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S-transferase (GST pi). MAb AGST I can inhibit 50-70% of GST pi enzymatic activity and reacts with a 3-dimensional epitope which includes a putative glutathione binding site on GST pi. A sandwich enzyme-immunoassay established using MAb AGST I and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST pi levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro- and chondrosarcomas.

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