Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jan 15;174(1):176-80.
doi: 10.1016/0006-291x(91)90502-x.

A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification

F von Weizsäcker  1 , S Labeit, H K Koch, W Oehlert, W Gerok, H E Blum
Affiliations

A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification

F von Weizsäcker et al. Biochem Biophys Res Commun. .

Abstract

A simple and rapid method for the extraction, reverse transcription and PCR amplification of RNA from formalin-fixed, paraffin-embedded tissue is described. The procedure can be completed within 24 hours. In a first application of this method we detect human albumin mRNA in liver tissue, demonstrating the feasibility to retrospectively analyze gene expression and RNA viruses in fixed tissue.

PubMed Disclaimer

Publication types

LinkOut - more resources